antirabbit tomm40 antibody (Proteintech)
Structured Review

Antirabbit Tomm40 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antirabbit+tomm40+antibody/TOMM40+Antibody/pm38978225-30-27-32
Average 96 stars, based on 167 article reviews
Images
1) Product Images from "BRG1 promotes liver cancer cell proliferation and metastasis by enhancing mitochondrial function and ATP5A1 synthesis through TOMM40."
Article Title: BRG1 promotes liver cancer cell proliferation and metastasis by enhancing mitochondrial function and ATP5A1 synthesis through TOMM40.
Journal: Cancer biology & therapy
doi: 10.1080/15384047.2024.2375440
Figure Legend Snippet: Figure 7. BRG1 induces the expession of TOMM40 in HCC cells. (a) HepG2 cells were stained with TOMM40 and BRG1 antibody after BRG1 knockdown or overexpression. (b and c) the percentage of positive cells was analyzed by ImageJ software (n = 10). **p < .01, ***p < .001 vs siRNA-NC, ##p < .01, ###p < .001 vs pcDNA3.1-NC. (d) HuH-7 cells were stained with TOMM40 and BRG1 antibody after BRG1 knockdown or overexpression. (e and f) the percentage of positive cells was analyzed by ImageJ software (n = 10). ***p < .001 vs siRNA-NC, ##p < .01, ###p < .001 vs pcDNA3.1-NC.
Techniques Used: Staining, Knockdown, Over Expression, Software
Figure Legend Snippet: Figure 8. BRG1 induces the expession of TOMM40 in HCC cells. (a) HepG2 cells were immunostained with TOMM40 and BRG1 antibody after BRG1 knockdown or overexpression. Scale bar, 20 μm. (b and c) the relative fluorescence intensity was analyzed by ImageJ software (n = 10). ***p < .001 vs siRNA-NC, ##p < .01, ###p < .001 vs pcDNA3.1-NC. (d) HuH-7 cells were immunostained with TOMM40 and BRG1 antibody after BRG1 knockdown or overexpression. Scale bar, 20 μm. (e and f) the relative fluorescence intensity was analyzed by Image J software (n = 10). ***p < .001 vs siRNA-NC, ###p < .001 vs pcDNA3.1-NC.
Techniques Used: Knockdown, Over Expression, Fluorescence, Software
Figure Legend Snippet: Figure 9. BRG1 activates TOMM40/ATP5A1 pathway in HCC cells. (a) The expressions of BRG1, TOMM40 and ATP5A1 were analyzed using Western blotting after BRG1 knockdown or overexpression in HepG2 cells. (b-d) the relative intensities of BRG1, TOMM40 and ATP5A1 were determined using ImageJ software (n = 3). **p < .01, ***p < .001 vs siRNA-NC, ##p < .01, ###p < .001 vs pcDNA3.1-NC. (e) The expressions of BRG1, TOMM40 and ATP5A1 were analyzed using Western blotting after BRG1 knockdown or overexpression in HuH-7 cells. (f-h) the relative intensity were determined using ImageJ software (n = 3). **p < .01, ***p < .001 vs siRNA-NC, ###p < .001 vs pcDNA3.1-NC. (i-l) the mRNA expressions level of BRG1, TOMM40 and ATP5A1 were analyzed using RT-qPCR.(n = 3). ##p < .01, ###p < .001 vs siRNA-NC, ***p < .001 vs pcDNA3.1-NC.
Techniques Used: Western Blot, Knockdown, Over Expression, Software, Quantitative RT-PCR
Related Articles
Blocking Assay:Article Title: BRG1 promotes liver cancer cell proliferation and metastasis by enhancing mitochondrial function and ATP5A1 synthesis through TOMM40. Article Snippet: Equal amounts of 30 μg protein were separated in an 8%~10% SDS-PAGE gel and transferred to a PVDF membrane (Cat#IPVH00010, Millipore). .. After blocking with 5% nonfat milk for 1 h at room temperature, the membranes were incubated with indicated primary antibodies: anti-rabbit BRG1 antibody (1:1000, 21634–1-AP, Proteintech, China), Incubation:Article Title: BRG1 promotes liver cancer cell proliferation and metastasis by enhancing mitochondrial function and ATP5A1 synthesis through TOMM40. Article Snippet: Equal amounts of 30 μg protein were separated in an 8%~10% SDS-PAGE gel and transferred to a PVDF membrane (Cat#IPVH00010, Millipore). .. After blocking with 5% nonfat milk for 1 h at room temperature, the membranes were incubated with indicated primary antibodies: anti-rabbit BRG1 antibody (1:1000, 21634–1-AP, Proteintech, China), |